Amino Acid Metabolism

Catabolism

1) Constant body turnover (75% reused for protein synthesis; 5% catbolized)

2) AA in excess of requirements

3) non-limiting AA are catabolized

4) AA for energy production ~20% of daily energy requirement (normal)

Deamination - removal of amino group as amonia

Transamination - Transfer of amino group from a compound to another

 

 

Ketogenic AA ® acetyl-CoA

1. leucine

2. isoleucine

3. phenylalanine

 

Gluconeogenic AA

All other AA - phenylalanine is both

 

Ammonia Toxicity:

Symptoms:

1. muscle tremors

2. slurred speech

3. blurred vision

4. ataxia, incoordination

5. tetany, violent spasms

6. comotose state

7. death - accute = 30 min.; or could take days

 

Urea Cycle

1. Ammonia detoxification

2. N for urea synthesis enters as NH3 (from deamination)

3. Requires 4 high energy phosphate bonds per urea molecule

4. Fate of urea in blood (BUN) - blood urea nitrogen

a. urine

b. rumen - via saliva

c. cecum

b. & c. are recycling

Birds - use uric acid as excretory product - requires glycine

Ruminants = recycle urea to rumen (horse to cecum??)